Hi Mark,
from what you write I expect you to be more or less a beginner in the audio world. I will write this from that point. As time goes and you learn more and find out about these things you will use your own ears and experience to change things.
So my suggestion is that you use 44.1kHz and 24 bit. There is rarely any really big difference going above 44.1kHz as sampling frequency. As for 24 bits of resolution I find that it saves a lot of trouble, not necessarily that it sounds very different from 16bits. The trouble you save is not having to be quite as exact with setting up levels.
Inside Cubase (at least the modern versions) are all 32 bit, but there is not much to think about there as that is done inside the program.
From the point of 44.1 / 24 you can start on making your recordings better. Positioning the mic and sound proofing your room is one of the things to work on.
Gunnar


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