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where to crank the gain up, Mixer, Interface, or on PC

Ok guys here is my setup Alesis 32 Mixer, MOTU 828 into my DAW. the 828 has gain knobs for all channels, but when I use these in conjunction with the trim knobs on the mixer I get a lot of noise. Should I just use the mixer preamps and not the MOTU's. It gets even more confusing when I am transfering my bands practice sessions from our Tascam 4-track > mixer > 828 > PC.

16bit trks to 24 for mixing: gain vs pain?

As a mixing assignment I'm presented with tracks recorded with a so-so 16 bit interface. They're not terrible, but of course they leave something to be desired.

What I'm asking is would it be benficial for me to convert them (internally via software) to 24 bit files, or perhaps just the stereo mix as a 24 bit file, before they ultimately get dithered back to 16?