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Latency; Pro Tools/MIDI

Hey guys, I'm having trouble recording with MIDI in Pro Tools(not only midi but mostly) When recording audio from a MIDI recording there is a latency of about, well it depends... I move the buffer size around from 1024/512 to 128, sometimes to 64. but as the session gets bigger, it gets to be a problem.
Is there any way of changing this without the hassle?

Juan

RAM - Lowest latency or Average?

I'm building my first DAW and ordering from Newegg. Just not certain whether it's beneficial to get the lowest latency or the average one. Both are compatible with the board from ASUS...because I've already checked. I will be loading GPO, SampleTank 2XL and maybe a few more samplers. I believe that these samplers are loaded into RAM, rather than DFD.

USB vs PCI latency (in cakewalk homestudio)

Hey, i'm new here, howzit?

I have an m-audio mobilepre (USB) that feeds my cakewalk homestudio. I find that latency tends to be a real pain. after every take, I have to go and re-align by hand so the timing matches up. would this be reduced significantly with a PCI soundcard? I don't want to spend the money if not.