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Do VST Instruments require actual MIDI Hardware?

Hello Everyone!
I am total newbee in digital mixing and will appreciate your patience with me. I was reading Nuendo2.0 and CubaseSX 1 manuals and they are not mentioning that actual hardware should be connected to VST host to utilize those VST's. Is there any chance to route the loop sample in to VST instrument for further manipulations within computer keyboard?